a) Background and b) positive signals across independent experiments as revealed by unstained and single‐peak rainbow beads, respectively. Machine performance across independent experiments. Matrix overlay of antigen expression between run 1 and run 5. Similar distribution of compensated data between independent experiments. The grey bar in the background represents the interquartile range of the distribution. Each dot represents a single independent experiment. b) Frequency of CD8 + T cells expressing a given antigen in 3 different healthy donors (HD). a) Gating strategy of the identification of CD8 + T cells in peripheral blood used in this study. 27‐parameter FACS is reproducible across independent experiments. Use the "Recalculate" menu command (cmd-=) to force a recalculation in the workspace display.Supplementary Figure 1. All recalculations are now done "lazy" - when needed. The "Recalculate" (Immediate/When Sample Loaded/When Necessary) preference has been removed.Layout Editor Tables that have a column for "Gate Name" - for sample-level nodes, the sample name is shown instead of the text "Ungated".Group Edit Dialog window properly initializes the comparator menu item.Allow CellQuest data files with zero events to be added to a workspace.Templates no longer give the error that the file was not properly closed.Templates with layout editor iteration that depends on keyword iteration and discrimination now work fully.When reading DiVa 3.0 data files, the progress bar better reflects how much is left to read and compensate.The Kinetics smoothing options are properly initialized in the kinetics window when re-opened.The Group Editor dialog window doesn\’t show funky characters for empty parameter stain names.The workspace comment column text is properly added to Layout Editor Tables.
![flowjo 10 error flowjo 10 error](https://docs.flowjo.com/wp-content/uploads/sites/6/2013/03/CCmodel1.png)
Edit menu garbage text (undo/redo) is fixed.
![flowjo 10 error flowjo 10 error](https://www.frontiersin.org/files/Articles/571321/fimmu-11-571321-HTML/image_m/fimmu-11-571321-g001.jpg)
![flowjo 10 error flowjo 10 error](https://i.ytimg.com/vi/Ux0S0g6-Fvs/maxresdefault.jpg)
This let\’s you add identifier information (or other information) found in the keywords as a real parameter, on which you can gate, etc. When you "concatenate" samples or gates, you can now choose (use the option-key when selecting "concatenate" from the menu) to create new parameters in the resulting data file, the contents of which are equal to keyword values from each of the samples.When creating a new Layout Editor Table, you can select (from the popup menu) an option to create a table that looks like the current Workspace (as much as possible) it automatically creates the appropriate columns.Layout Editor Tables can show the gate name with an "indented" option - to make it look like the Workspace display.There are new UI checkboxes in the overlay information dialog to set this Layout Editor overlay graphs can optionally show gates for all overlaid layers (not just top-most).Keyword Edit/Replace operations allow for searching for blank keywords (so you can replace blank keywords with any given replace value).